Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Estrogen and xenoestrogen modulated transcriptome in the pituitary of juvenile female Atlantic cod (Gadus morhua)

ABSTRACT: Estrogens regulate many reproductive processes in fish and other vertebrates. In fish, the pituitary and liver are among the main sites of action of estrogens in the reproductive brain-pituitary-gonadal-hepatic axis. Environmental contaminants with estrogenic compounds acting at these organs have the potential to interfere with the reproductive processes in fish. In this work, RNA-seq analysis was performed to map estrogen and xenoestrogen target genes in the juvenile female Atlantic cod (Gadus morhua) after 72 h exposure to estrogenic compounds ethynylestradiol (EE2), and the xenoestrogens bisphenol A (BPA) and methoxychlor (MXC). Our results show that, in the pituitary, EE2 and the xenoestrogens modulated many genes and pathways related to reproduction (such as hormone synthesis, hormonal signaling), metabolic homeostasis, and cell differentiation and proliferation. The xenoestrogens BPA and MXC had similar effect as EE2 on the top differentially expressed genes, suggesting their potential to disrupt pituitary functions.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Gadus morhua

SUBMITTER:

PROVIDER: E-MTAB-12762 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP145592

REPOSITORIES: biostudies-arrayexpress

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Project description:17alpha-ethinylestradiol (EE2) is one of the most potent estrogens that have the ability to interfere with the endocrine system of fish. The objective was to investigate the effects and mechanisms of action caused by 60 days of dietary exposure to 0.2 mg EE2/kg and 0.07 mg EE2/kg feed in female largemouth bass (LMB) during the reproductive season. Microarrays and pathway analyses were performed on hepatic tissues to identify genes and biological processes altered in female LMB by EE2 exposure. The hypothesis was that the two concentrations of EE2 would produce dose-response changes in sensitive genes. Body and ovary weights were measured and blood was collected for measurement of plasma steroid hormones (17beta-estradiol (E2), testosterone (T)) and vitellogenin (VTG) using ELISA. The 0.2 mg EE2/kg feed exposure reduced the gonadosomatic index (GSI) by 75%, and plasma levels of E2 and T were reduced by over 90%. Plasma VTG was increased by approximately 100% (from 4 to 8mg/ml) by the 0.2 mg/kg treatment. T levels, from the 0.07 mg EE2/kg feed, reduced GSI by approximately 30% and circulating E2 and T by ~80% but did not affect VTG concentrations. We found 1,594 and 1,165 genes were significantly affected (p<0.05) by the 0.07 mg EE2/kg feed and 0.2 mg EE2/kg feed, respectively. Gene ontology (GO) analysis revealed that there were different biological processes regulated by the two concentrations of EE2. Pathway analysis showed that the 0.07 mg EE2/kg feed exposure caused differential regulation of genes associated with fatty acid biosynthesis and glycolysis, indicating some metabolic effects. In contrast, the 0.2 mg EE2/kg feed exposure altered transcription of genes involved in immune response and apoptosis, suggesting a toxic response at this concentration. These results suggest that the two concentrations demonstrated distinct physiological responses, with the higher concentration inducing complete endocrine disruption in LMB. These findings demonstrate the usefulness of microarrays to identify possible biomarkers and modes of toxic action to dietary exposure in LMB. Two concentrations of EE2 would produce dose-response changes in sensitive genes. Female LMB were fed 5 days per week for 60 days with floating pellets that contained 0.07 or 0.2 mg/kg of EE2.

Dataset Information

Estrogen and xenoestrogen modulated transcriptome in the pituitary of juvenile female Atlantic cod (Gadus morhua)

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