Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of S. cerevisiae (BY4741) treated with Lanosterol against untreated controls in the presence and absence of alpha-factor


ABSTRACT: We have performed paired-end RNA sequencing on S. cerevisiae (BY4741). Lanosterol (LST) was predicted as a potential modulator of the Ste2 (GPCR)-mediated pheromone-induced cell death using Gcoupler. The cells were grown at 30°C at 200 r.p.m. in YPD medium in biological duplicates in the presence or absence of lanosterol (LST) at a final concentration of 1 μM. Post-metabolite treatment, the cells were either treated or untreated with the α-factor at a final concentration of 30 μM for 2 hours at desired growth conditions. Post these steps, the cells were harvested, and RNA was isolated, and sequenced.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER:  

PROVIDER: E-MTAB-12992 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP147620

REPOSITORIES: biostudies-arrayexpress

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