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Whole blood RNA-Sequencing from an Influenza H3N2 human challenge model

ABSTRACT: The Influenza H3N2 human challenge model has been described in detail previously5. Briefly, 20 healthy volunteers were inoculated intranasally with a standardized dose of Influenza A virus (A/Belgium/4217/2015(H3N2)) under quarantine conditions. From 24 hours after inoculation, virus was quantified by PCR in nasal lavage samples obtained at 12 hourly intervals. Participants were ascertained to have replicative viral infection if found to have consecutive positive PCR tests at least 24 hours after challenge. Blood samples for RNA sequencing were collected into PAXgene tubes before virus challenge and days 1, 2, 3, 7, 10, 14, and 28 after challenge. These were stored at -80°C until processing

INSTRUMENT(S): R, Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Myrsini Kaforou

PROVIDER: E-MTAB-13041 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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