SHAPE-Seq of human U2 snRNA and U4 pre-snRNA

ABSTRACT: Spliceosomal snRNA are key components of small nuclear ribonucleoprotein particles (snRNPs), the building blocks of the spliceosome. The biogenesis of snRNPs is a complex process involving multiple cellular and subcellular compartments, the details of which are yet to be described. In short, the snRNA is exported to the cytoplasm as 3‘-end extended precursor (pre-snRNA), where it acquires a heptameric Sm ring. The SMN complex which catalyses this step, recruits Sm proteins and assembles them around the pre-snRNA at the single stranded Sm site. After additional modification, the complex is re-imported into the nucleus where the final maturation step occurs. Our modeling suggests that during the cytoplasmic stage of maturation pre-snRNA assumes a compact secondary structure containing Near S

INSTRUMENT(S): NextSeq 500, NextSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER:  

PROVIDER: E-MTAB-13248 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP150188

REPOSITORIES: biostudies-arrayexpress