Single cell RNAseq of peripheral CD4+ T cells in the context SARS-CoV-2 infection

ABSTRACT: CD4+ T cells play a fundamental role in host defenses against viruses by orchestrating B cell development and/or by directly targeting pathogens. This dataset aimed at understanding the molecular profile of CD4+ T cells in the context of an acute and severe SARS-CoV-2 infection. The analyzed patient was recruited at Henri Mondor University Hospital (AP-HP, Paris France) in March 2020, and required oxygen as treatment. Peripheral (CD14-CD56-CD123-CD19-CD3+CD4+CD45RA-PD-1high) T cells were FACS-sorted using a Sony MA900 in PBS/0.08% FCS. 1.105 cells were obtained and 20000 were loaded in the 10x Chromium Controller to generate single-cell gel-beads in emulsion. The scRNA-seq libraries were generated using the Chromium Next GEM Single Cell V(D)J Reagent Kit v.1.1 with Feature Barcoding (10x G

INSTRUMENT(S): Illumina NovaSeq 6000, Sony MA900

ORGANISM(S): Homo sapiens

SUBMITTER: Manfroi Benoît 

PROVIDER: E-MTAB-13321 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress