Project description:Identification of target genes for the transcription factor HSFA4A in Arabidopsis thaliana. Two weeks-old Col-0 wild-type and transgenic plants overexpressing the HSFA4A transcription factor under the estradiol-inducible promoter lexA (HSFA4Aox2) were treated by 5µM estradiol with or without 1mM H2O2 for 6h in liquid 0.5 MS medium and the isolated RNA samples were used for RNAseq analysis.
Project description:To identify targets of the C2H2 zinc finger protein ZINC 34 FINGER OF ARABIDOPSIS THALIANA 14 (ZAT14, AT5G03510), we used an estradiol-inducible system for inducible expression of ZAT14, comparing gene expression in estradiol-treated plants and mock-treated 5-day old seedlings 8 hours after estradiol or mock treatment.
Project description:Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Inducible silencing of MKIP1 by estradiol-treatment results in pale newly emerged leaves. Here we analysed the proteome of these leaves from two independent plant lines by LC-MS/MS using data-independent acquisition. Estradiol-treated wild-type (Col-0) plants and a line in which the plastidial ribosomal subunit uL4c was silenced served as controls. Data was analysed using DIA-NN via the FragPipe platform.
Project description:A search for MTF-1 target genes and cadmium-inducible genes in the adult liver was performed by comparing liver-specific transcription in Mtf1 conditional knockout mice and control littermates.
Project description:ZFP3, a nuclear C2H2 zinc finger protein acts as a negative regulator of ABA- suppressed germination. Regulated over-expression of ZFP3 and closely related ZFP1, ZFP4, ZFP6 and ZFP7 confers ABA insensitivity to seed germination while the zfp3,zfp4 double mutant displays enhanced ABA susceptibility. Reduced expression of a large set of ABA-induced genes such as RAB18 and transcription factors ABI3, ABI4, ABI5 and RGL2 in ZFP3ox seedling suggests that ZFP3 indeed negatively regulates ABA signaling. Analysis of ZFP3ox plants revealed multiple phenotypic alterations such as semidwarf growth habit, defects in fertility and enhanced sensitivity of hypocotyl elongation to red but not to far-red or blue light. Analysis of genetic interactions with phytochrome and abi mutants suggested that ZFP3 amplifies red light signals perceived by photoreceptors other than phyB, and controlled by ABI5 downstream of ZFP3. Comparison of ZFP3 overexpressing and wild type Arabidopsis seedlings
Project description:Mutation of MTF in Arabidopsis increases Agrobacterium-mediated transformation susceptibility. Being a putative transcription factor, different genes controlling transformation may be regulated by MTF. We used microarrays to detail the global programme of gene expression and to identify genes that play a role in transformation. Root samples from mutants MTF1-1 and MTF1-4, along with the wild-type, Col0, were collected after 12 days of growth in B5 liquid medium. RNA was extracted from three biological replicates, with 20 plants in each replicate, and used for hybridization to Affymetrix ATH1 microarrays.
Project description:Sequence-specific transcription factor WRKY75 is highly responsive to reactive oxygen species on transcriptional level in the rosettes of Arabidopsis thaliana. In addition, it acts in developmental responses, acquisition of nutrients, and in stress responses. In the root, WRKY75 is a repressor of root hair formation, it regulates the phosphate starvation response, and the response to certain pathogens. In order to find the target genes of WRKY75, the effects of estradiol-inducible overexpression of WRKY75 on transcriptome was studied using RNA-seq. Two independent inducible WRKY75 overexpressor lines (N2102362 and N2102363) were treated with estradiol for 3 hours and four biological replicates, five plant per replicate, were used for RNA-seq. As a control, wild type Col-0 was treated with estradiol or water for 3 hours and three biological replicates, five plant per replicate, were used for RNA-seq.
Project description:Plants as non-mobile organisms constantly integrate varying environmental signals to flexibly adapt their growth and development. Local fluctuations in water and nutrient availability, sudden changes in temperature or other abiotic and biotic stresses can trigger changes in the growth of plant organs. Multiple mutually interconnected hormonal signaling cascades act as essential endogenous translators of these exogenous signals in the adaptive responses of plants. Although the molecular backbones of hormone transduction pathways have been identified, the mechanisms underlying their interactions are largely unknown. Here, using genome wide transcriptome profiling we identify an unknown auxin and cytokinin cross-talk component; SYNERGISTIC ON AUXIN AND CYTOKININ1 (SYAC1), whose expression in roots is strictly dependent on both of these hormonal pathways. We show that SYAC1 is a component of secretory pathway, whose enhanced activity interferes with deposition of cell wall components and can fine-tune organ growth and sensitivity to soil pathogens
Project description:This RNAseq experiment aims at determining the reprogramming of gene expression upon loss of K63 polyubiquitin chain formation. Twelve-day-old plants expressing a beta-estradiol inducible artificial microRNA targeting the two plant E2 enzymes catalyzing K63 polyubiquitin chain formation were mock-treated or beta-estradiol-treated (5microM). Total RNAs from whole seedings were extracted and subjected to RNA-seq analyses. Genes that are differentially regulated between non-treated (uninduced) and treated (induced with beta-estradiol) were identified.