Overexpression of non-degradable SAS-6 KEN box mutant in RPE-1 cells

ABSTRACT: Stable clones of RPE-1 cells expressing tetracycline-inducible wild type SAS-6 or SAS-6ND were obtained via lentiviral gene transduction with the pLVX tet-on Advanced inducible gene expression system (Clontech). Stable expressors were derived by selection with 5 μg/mL puromycin (Sigma-Aldrich, UK). Doxycycline 1 microgram/mL was added to growth media for 6 days to induce SAS-6 expression. RNA was isolated from RPE-1 cells using the RNeasy mini kit (Qiagen, CA) according to the manufacturer’s protocol. Gene expression was profiled using GeneChip™ Human Transcriptome Array 2.0.

ORGANISM(S): Homo sapiens

SUBMITTER: NICOLAOS PALASKAS 

PROVIDER: E-MTAB-13783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress