Gene expression profiling of siderophore-drug resistant mutant of Pseudomonas aeruginosa PAO1

ABSTRACT: The aim of the experiment was to identify genes differentially expressed between the susceptible wild type strain P. aeruginosa PAO1 (PT5) and a mutant resistant to a drug-siderophore conjugate, in order to obtain information on the resistance mechanism(s). A mutant of PT5 able to grow at 4 mg/l BAL30072, a drug-siderophore conjugate, was selected in vitro . The susceptible wild type strain PT5 and the mutant (BAL6) were grown in LB medium and the mutant also in the presence of 4 mg/l BAL30072 to mid-exponential growth phase (OD600 =2) in triplicate cultures. RNA was extracted using the RNeasy Kit (Qiagen). A total of nine Affymterix P. aeruginosa arrays were hybridized (one for each replicate) under standard conditions.

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Olivier Schaad 

PROVIDER: E-MTAB-1381 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress