RNA-seq of germinating barley embryos in control and ABA

ABSTRACT: To address the lack of information on the function of CBC in the ABA signaling pathway during seed germination in barley, we employed TILLING to identify mutants of the genes hvcbp20.ab and hvcbp80.b and subsequently created the double mutant hvcbp20.ab/hvcbp80.b. We found that mutations in both CBC subunits resulted in a unique ABA response that differed from that of single mutants. Through transcriptomic analyses, we identified differential gene expression and alternative splicing patterns. Notably, the hvcbp20.ab/hvcbp80.b double mutant exhibited altered AS regulation and significant changes in brassinosteroid signaling following ABA exposure. Seeds were sterilized in 20% sodium hypochlorite solution for 20 min and washed with sterilized water for 5 min. They were then placed in Petri d

INSTRUMENT(S): Illumina NovaSeq 6000, -

ORGANISM(S): Hordeum vulgare

SUBMITTER: Agata Daszkowska-Golec 

PROVIDER: E-MTAB-13989 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress