Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from 12-day old Arabidopsis germinants

ABSTRACT: Seed germination of a terrestrial plant constitute dynamic changes in various physiological processes related to growth and development. These physiological processes can be affected by various abiotic and biotic stressors. Here we looked at how the two commonly used nanoparticles, nano-titania (TiO2) and nano-ceria (CeO2) can impact the underlying mechanisms associated with germination at genome level. We used microarrays to detail the global programme of gene expression underlying various physiological processes associated with growth and development, and identified distinct classes of up-regulated genes during this process. 12 day old Arabidopsis germinants were selected for RNA extraction and hybridization on Affymetrix microarrays. Arabidopsis seeds were individually exposed to 500mg/L concentration of nano-titania and nano-ceria for 12 days, followed by extraction of RNA for Microarray analysis. 0.1M KCl was used as control for nano-titania, whereas sterilized millipore water was used as control for nano-ceria.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Laxminath Tumburu 

PROVIDER: E-GEOD-59809 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Phenotypic and genomic responses to titanium dioxide and cerium oxide nanoparticles in Arabidopsis germinants.

Tumburu Laxminath L   Andersen Christian P CP   Rygiewicz Paul T PT   Reichman Jay R JR  

Environmental toxicology and chemistry 20141103 1

The effects of exposure to nanoparticles of titanium dioxide (nano-titanium) and cerium oxide (nano-cerium) on gene expression and growth in Arabidopsis thaliana germinants were studied by using microarrays and quantitative real-time polymerase chain reaction (qPCR), and by evaluating germinant phenotypic plasticity. Exposure to 12 d of either nano-titania or nano-ceria altered the regulation of 204 and 142 genes, respectively. Genes induced by the nanoparticles mainly include ontology groups an  ...[more]

Similar Datasets

Transcriptomics Expression data from 29-day old Arabidopsis plants
2016-05-31 | E-GEOD-80461 | biostudies-arrayexpress
Transcriptomics Expression data from 12-day old Arabidopsis germinants
2014-07-29 | GSE59809 | GEO
Transcriptomics Genome-wide analysis of desiccation tolerance-related genes in Arabidopsis seeds
2013-06-21 | E-GEOD-30853 | biostudies-arrayexpress
Proteomics Tandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.
2016-02-09 | PXD003352 | Pride
Transcriptomics Expression data from 29-day old Arabidopsis plants
2016-05-31 | GSE80461 | GEO
Transcriptomics The embryonic leaf identity gene FUSCA3 also regulates vegetative phase transitions by negatively regulating ethylene action in Arabidopsis
2011-12-11 | E-MEXP-3465 | biostudies-arrayexpress
Transcriptomics RNAseq of 4 day old etiolated Arabidopsis thaliana Ler and gin2-1 seedlings grown in darkness at 18C
2023-05-24 | E-MTAB-7654 | biostudies-arrayexpress
Transcriptomics Gene expression in pif3 mutant under dark and in wt under Red 1h and Red 18h
2012-01-03 | E-GEOD-30030 | biostudies-arrayexpress
Proteomics Quantitative proteomics reveals dual effects of calcium on radicle protrusion in soybean
2020-10-19 | PXD021238 | Pride
Transcriptomics Transcriptomic analysis of regulatory gene expression profiles by NF-YCs, and RGL2 under paclobutrazol (PAC) condition
2016-07-25 | E-GEOD-84753 | biostudies-arrayexpress