Targeted PCR amplification and sequencing of reporter constructs from stage 6 drosophila embryos
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ABSTRACT: Targeted PCR amplification and sequencing of a reporter construct from a nos-phiC31; attP40 line of Drosophila stage 6 embryos. Flies carried two copies of one of 25 different enhancer reporter assays. The region amplified by PCR contains the enhancer and 10X cell barcodes required for analysis. Three samples were generated from the three cDNAs generated during the 10X 3'end scRNA-seq experiment. The complementary scRNA-seq datasets are available here:
INSTRUMENT(S): Illumina NovaSeq X
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Baptiste Alberti
PROVIDER: E-MTAB-14445 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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