Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Clariom S Microarray from gWAT RNA of female and male offspring of T3 treated and control dams

ABSTRACT: This study analysed the effect of maternal hyperthyroidism on the offspring’s white adipose tissue function. Hyperthyroidism during pregnancy was induced in wild type female C57BL/6NCrl (Charles River, Germany) at the age of three to four month with 0.5 mg/L T3 (3,3’,5-Triiodo-L-thyronine, T6397, Sigma Aldrich, Germany) in drinking water with 0.01% BSA from day of positive plug check until gestational day 18 (= day before birth). Adipose tissue samples were collected at the day of sacrifice (male offspring: 5-6 months, female offspring: 6-7 months), weighted, snap frozen on dry ice, and stored at -80 °C until nucleic acid extraction. Sex-specific gene expression profiling in the gWAT samples from the offspring of maternal T3 treatment experiment was performed using GeneChip Clariom S arrays (Affymetrix, Germany).

ORGANISM(S): Mus musculus

SUBMITTER: Luise Müller

PROVIDER: E-MTAB-14515 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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