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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome Profiling of UCL93 Kidney Epithelial Cells and CRISPR-Cas9 Knockouts for PKD1 and BICC1 Using Total RNA-Seq

ABSTRACT: Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the most common hereditary cause of kidney failure, resulting from germline mutations in PKD1 and PKD2 in over 90% of cases. Polycystin-1 (PC-1), a cell surface receptor linked to focal adhesion proteins, plays a critical role in regulating cell-matrix and cell-cell interactions. This study explores the hypothesis that the disrupted actin phenotype observed in ADPKD may be influenced by F-actin binding and the translational regulation of actin-associated proteins mediated by a PC1-BICC1 complex. We examined whether actin-associated pathways are deregulated in UCL93 kidney epithelial cells and CRISPR-Cas9 knockout models for PKD1 and BICC1. Total RNA was extracted for gene expression analysis using total RNA-Seq, and the data were utilized to identify deregulated pathways in the knockout models.

INSTRUMENT(S): Agilent 2100, Illumina HiSeq 2500, ShARC

ORGANISM(S): Homo sapiens

SUBMITTER: Manoj Valluru

PROVIDER: E-MTAB-14624 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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