Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of perirenal adipose tissue of Assaf suckling lambs whose dam were subjected to a dietary protein restriction at prepubertal age

ABSTRACT: Samples of perirenal fat tissue from 8 Assaf breed suckling lambs. These animals were offspring of dams subjected to a Nutritional Protein Restriction (NPR) at the prepubertal age as described by Mateo et al. (2023) [doi: 10.1016/j.livsci.2023.105173]. These animals were reared in the same batch that other 8 Assaf suckling lambs obtained from Control dams of the NPR experiment for which RNA-Seq samples are already available (see E-MTAB-11839 for RNA-Seq data of Control lambs). These 8 NPR animals were selected from a larger group of 17 NPR suckling lambs for which carcass traits were measured. The 8 selected lambs were those from the 17 initially considered lambs showing the highest and the lowest values , from the larger group, for the percentage of kidney knob and channel fat relative to the right half carcass weight (KKCF). Hence, considering the values for this trait, we defined the High-KKCF group (n = 4; average: 3.26 ± 0.11) and the Low-KKCF group (n = 4; 1.68 ± 0.25), respectively.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Ovis aries

SUBMITTER:

PROVIDER: E-MTAB-15012 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-11839

REPOSITORIES: biostudies-arrayexpress

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Project description:A summary of the work associated to these microarrays is the following: Diet plays a role in the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding 80:20 isomer mix of c9,t11 and t10,c12 CLA, respectively. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life. With all these concepts in mind, we determined the expression profile of Mesentheric Lymph Nodes (MLN) from animals supplemented with CLA during gestation and suckling through dam’s milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) with the aid of the specific GeneChip ® Rat Genome 230 2.0 (Affymettrix). Bioinformatic analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 123 genes differentially expressed in all three dietary approaches. Generation of a Biological Association Network (BAN) evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2) actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR. We conclude that Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on lymphoproliferation and mucosal immune responses in early life. The aim of our study was to evaluate, by using whole genome microarrays, the effects of dietary supplementation with an 80:20 isomer mix of c9,t11 and t10,c12 CLA, respectively, on Mesentheric Lymph Nodes gene expression, during gestation and/or suckling. Three experimental approaches were conducted to assess the effects of CLA supplementation on I) pups from dams fed with 1% CLA diet during the two weeks of gestation and throughout the suckling period (Group A). These pups received CLA through the dam’s milk during suckling (Total Period of CLA Supplementation (TPS) 5 wk); II) pups from dams fed with 1% CLA diet during gestation and standard diet during suckling. These pups were CLA-supplemented daily during suckling by oral gavage (TPS 5 wk) (Group B); III) pups from dams fed standard diet during gestation and suckling and receiving CLA by daily oral gavage throughout the suckling period (TPS 3 wk) (Group C). Group D, pups from dams fed standard diet throughout the study, constituted the reference diet group (TPS 0 wk). Triplicate samples were hybridized for each experimental condition (12 samples in total). The samples provided were analyzed using the specific software GeneSpring GX.

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RNA-Seq of perirenal adipose tissue of Assaf suckling lambs whose dam were subjected to a dietary protein restriction at prepubertal age

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