Comparison of Microarray Synthesis with and without Incorporating Debranching/Capping Step via Gene Expression Analysis
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ABSTRACT: Gene expression microarrays require synthesizing a vast number of unique 60mer high-fidelity DNA probes to achieve strong signal intensity and low background noise, which are essential for accurate data interpretation. Due to the high number of coupling cycles involved in the synthesis, these DNA probes are susceptible to degradation, especially if synthesized in the absence of a debranching/capping step. This study compared gene expression microarrays synthesized with and without incorporating the debranching step to evaluate their impact on probe quality and the detection sensitivity for the genes expressed above the background levels.
INSTRUMENT(S): Innopsys Innoscan
ORGANISM(S): Homo sapiens
SUBMITTER: Santra Santhosh
PROVIDER: E-MTAB-15163 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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