Brassinosteroids Enhance Barley De-Acclimation Tolerance via Modulation of Chloroplast- Associated Gene Expression
Ontology highlight
ABSTRACT:
INSTRUMENT(S): BGISEQ-500
ORGANISM(S): Hordeum vulgare
SUBMITTER: Magdalena Wójcik-Jagła
PROVIDER: E-MTAB-15311 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:The aim of this study was to identify genetic mechanisms of tolerance to active de-acclimation in winter wheat by comparing four tolerant and four susceptible accessions (cultivars and advanced breeding lines). The plants were kept in controlled conditions. 21 days after sowing, the plants were cold acclimated (3 weeks, 4/2 °C, day/night temperature; photoperiod of 10/14 h). Cold acclimation was followed by 7 days of de-acclimating conditions, mimicking a mid-winter warm spell: 12/5°C, day/night temperature; photoperiod of 10/14 h). The fragments of leaves were sampled in three biological replicates (three different plants of the same accession) in three timepoints: just before cold-acclimation (CA-0), after cold-acclimation (CA-21), and after de-acclimation (DA-28). Total RNA samples were sequenced on Illumina NovaSeq 6000 platform in PE150 bp mode. Differences in expression for a given accession were tested between all time-points, averaging the results for three biological replicates. It has been confirmed that active de-acclimation is not simply the inverse of cold acclimation, and that susceptible forms show significantly more changes in expression as a result of de-acclimation than tolerant forms. This provides further support for the hypothesis that tolerance to de-acclimation consists mainly of the absence or weakest response to temperature rise. Among the genes whose expression was significantly altered as a result of de-acclimation, a significant group were genes related to the defence response to stress. In wheat particularly plant hormone related genes, and plant hormones, seem to be playing a crucial role in the response to active de-acclimation, especially ABA and SA. Our results also suggest that in wheat the period of cold-acclimation might be decisive in the later active de-acclimation tolerance.
2026-03-16 | E-MTAB-16628 | biostudies-arrayexpress
Project description:During cold acclimation plants increase their freezing tolerance in response to low non-freezing temperatures. This is accompanied by many physiological, biochemical and molecular changes that have been extensively investigated. In addition, many cold acclimated plants become more freezing tolerant during exposure to mild, non-damaging sub-zero temperatures. There is hardly any information available about the molecular basis of this adaptation. However, Arabidopsis thaliana is among the species that acclimate to sub-zero temperatures. This makes it possible to use the molecular and genetic tools available in this species to identify components of sub-zero signal transduction and acclimation. Here, we have used microarrays and a qRT-PCR primer platform covering 1880 genes encoding transcription factors to monitor changes in gene expression in the accessions Columbia-0, Rschew and Tenela during the first three days of sub-zero acclimation at -3°C. The results indicate that gene expression during sub-zero acclimation follows a tighly controlled time-course. Especially AP2/EREBP and WRKY transcription factors may be important regulators of sub-zero acclimation, although the CBF signal transduction pathway seems to be less important during sub-zero than during cold acclimation. Globally, we estimate that approximately 5% of all Arabidopsis genes are regulated during sub-zero acclimation. Particularly photosynthesis-related genes were down-regulated and genes belonging to the functional classes of cell wall biosynthesis, hormone metabolism and RNA regulation of transcription were up-regulated. Collectively, these data provide the first global analysis of gene expression during sub-zero acclimation and allow the identification of candidate genes for forward and reverse genetic studies into the molecular mechanisms of sub-zero acclimation. We used whole genome microarrays to monitor changes in gene expression in the Arabidopsis thaliana accessions Columbia-0, Rschew and Tenela during three days of acclimation to sub-zero temperature at -3°C after cold acclimation Plants from Arabidopsis thaliana accessions Columbia-0, Rschew and Tenela were cold acclimated at 4°C for two weeks. Detached leaves were then sub-zero acclimated at -3°C for 8 h, 1 d or 3 d at -3°C. Leaves of cold acclimated plants and sub-zero acclimated leaves were collected for RNA extraction and hybridization on Affymetrix ATH1 microarrays in order to explore temporal transcriptome changes during sub-zero acclimation. For each sample total RNA was isolated from a pool of three leaves from three different plants. The experiment was performed in three idenpendent biological replicates.
2014-03-12 | E-GEOD-55835 | biostudies-arrayexpress
Project description:Dynamic acclimation of photosynthesis plays an important role in increasing plant fitness under variable light environments. Acclimation is mediated by a glucose-6-phosphate/phosphate translocator, GPT2. This study examined whether plants lacking GPT2, which are defective in acclimation to increases in light, are more susceptible to oxidative stress. To understand this, we used the model plant of Arabidopsis thaliana (accession Wassilewskija-4 (Ws-4)) and compared this to mutants lacking GPT2. Plants were grown at low light (100 μmol m−2 s−1) for 7 weeks. For acclimation experiments, a set of plant from low light was transferred to 400 μmol m−2 s−1 for 7 days. Microarray analysis showed that gpt2 plants showed a greater induction of stress related genes relative to WT.
2021-03-24 | E-MTAB-10282 | biostudies-arrayexpress
Project description:In this study we used the Affymetrix Barley 1 GeneChip to investigate transcriptome responses of barley cv. Morex to low temperature, including triplicated measurements of cold, freeze/thaw cycles and de-acclimation over 33 days. Experiment Overall Design: Plants were grown at 20ºC for seven days and subject to a symmetrical cycle of acclimation, cold, freeze-thaw, and deacclimation. Chilling began by decreasing the temperature overnight from 20ºC to 4ºC at a rate of 1.3ºCâ¢h-1 and maintaining temperatures of 4 ºC in the day and 2ºC at night for 5 days. Freeze-thaw cycling lasted 12 days with day temperatures of 4ºC and night temperatures gradually decreasing from -2ºC the first night to -4ºC for three nights and -10ºC for four nights, then recovering to -4ºC for three nights and -2ºC for one night. This treatment was designed to allow daily freeze-thaw cycling and protein synthesis. Chilling conditions (4ºC day, 2ºC night) were resumed for five days, followed by deacclimation with increasing temperature to 20ºC overnight and maintaining for three days. Sampling was done at four different times, each at the 11th hour of light to avoid circadian effects: 1) before chilling treatment, 2) five days after initiation of chilling treatment, 3) eight days into freeze-thaw treatment and 4) three days into de-acclimation.
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Project description:Heat stress is the primary obstacle for landscape application of azalea in hot-summer areas. Heat acclimation can confer acquired thermotolerance to R. hainanense even after a long recovery. The mechanisms and key factors involved in the heat acclimation memory were investigated.
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Project description:Stress acclimation is an effective mechanism that plants acquired for adaption to dynamic environmental conditions. After undergoing cold acclimation, plants become more tolerant to cold stress. In order to understand the mechanism of cold acclimation, we performed a systematic, comprehensive study of cold response and acclimation in Cassava (Manihot esculenta), a staple crop and major food source in the tropical regions of the world. We profiled mRNA genes and small-RNA species, using next generation sequencing, and performed an integrative analysis of the transcriptome and microRNAome of Cassava across the normal condition, a moderate cold stress at 14M-BM-0C, a harsh stress at 4M-BM-0C after cold acclimation at 14M-BM-0C, and a cold shock from 24M-BM-0C to 4M-BM-0C. Two results from the analysis were striking. First, the moderate stress and cold shock, despite a difference of 10M-BM-0C between the two, triggered comparable degrees of perturbation to the transcriptome; in contrary, further harsh stress after cold acclimation resulted in a much smaller degree of transcriptome variation. Second and more importantly, about two thirds of the up- or down-regulated genes after moderate stress reversed their expression to down- or up-regulation, respectively, under harsh stress after cold acclimation, resulting in a genome-wide rewiring of regulatory networks. MicroRNAs, which are key post-transcriptional gene regulators, were major players in this massive rewiring of genetic circuitry. Further, a function enrichment analysis of the perturbed genes revealed that cold acclimation helped the plant to develop immunity to further harsh stress by exclusively inducing genes with functions of nutrient reservoir; in contrast, many genes with functions of viral reproduction were induced by cold shock. Our study revealed, for the first time, the molecular basis of stress acclimation in plants, and shed lights on the role of microRNA gene regulation in cold response and acclimation in Euphorbia. Three organs/tissues (folded leaf, fully expanded leaf and roots) of Cassava cultivar SC124 harvested at 6h, 24h and 5d for three cold treatments of CA, CCA and CS, for gene expression profiling at the stages of initial response, secondary response, and functional adaption to cold stresses. Total RNA of each sample was isolated individually, and then pooled with an equal amount from each sample into one for profiling. As a result, four mRNA libraries and four small-RNA libraries, corresponding to the conditions of CA, CCA, CS and NC, were constructed.
2014-08-18 | E-GEOD-52176 | biostudies-arrayexpress
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