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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ScRNA-Seq of human MAIT cells sorted from PBMCs of pancreatic ductal adenocarcinoma patients and healthy controls

ABSTRACT: As pancreatic ductal adenocarcinoma (PDAC) is the deadliest solid cancer, and new immunological therapies have only marginally improved patient survival, we aimed at better understainding the immunological fingerprint of this disease. PDAC development involves inflammatory reprogramming and bacterial colonization, conditions that influence the adaptation of mucosa-associated invariant T cells (MAIT). We investigated MAIT signatures in patients with PDAC, chronic pancreatitis (CP), a risk factor for PDAC, and healthy donors (HD) using high-dimensional single-cell techniques, complemented by serum proteomics and multicolor histology. In our manuscript, we identify for the first-time human effector MAIT1 cells likely involved in protecting against PDAC. We characterize the transcriptional, phenotypic, and metabolic signatures of human MAIT1 compared to MAIT17-like cells, their adaptation to the PDAC inflammatory microenvironment, and provide insights into mechanisms underlying MAIT1-mediated protection against PDAC.

INSTRUMENT(S): BD Rhapsody, BD Rhapsody Sequence Analysis Pipeline, Illumina NovaSeq X, Aurora Cytek Cs 5L

ORGANISM(S): Homo sapiens

SUBMITTER: Teresa Ruckenbrod

PROVIDER: E-MTAB-15362 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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