A Modular Bioinstructive Platform For Additive-Free Control of Stem Cell Differentiation and Patterning

ABSTRACT: Recreating 3D bone formation in vitro without biochemical inducers remains a longstanding challenge in preclinical testing. We present a modular, bioinstructive platform based on polylactic acid microparticles with controlled dimpled surface features that direct mesenchymal stem cell differentiation through endogenous mechanotransduction, establishing a mechanistically validated, additive-free platform. These dimpled 3D topographical cues drive cytoskeletal reorganisation and induce osteogenesis via canonical Hedgehog signalling. RNA-Seq revealed early significant upregulation of cytoskeletal components and osteochondral transcription factors, including runt-related transcription factor 2 (RUNX2) and SRY-box transcription factor 9 (SOX9), followed by activation of the insulin growth factor

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Leo Zeef 

PROVIDER: E-MTAB-15393 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress