Unknown,Transcriptomics,Genomics,Proteomics

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Effects of root-specific deficiency of each cytokinin receptor AHK2, AHK3, or AHK4 on genome-wide gene expression in shoots and roots of Arabidopsis thaliana

ABSTRACT: Trans-zeatin (tZ)-type cytokinin (CK) positively regulates leaf size and number by translocating from root to shoot in response to nitrate signals. Our preceding study suggested that CK perception in the root may influence the long-distance transport of tZ-type CKs from root to shoot. However, the underlying mechanism and physiological significance remain unclear. Therefore, we generated grafted plants with the wild-type Col-0 (WT) as the scion and either WT or one of three CK receptor mutants - Arabidopsis histidine kinase (ahk) 2, ahk3, or ahk4 - as the rootstock (scion/rootstock: WT/WT, WT/ahk2, WT/ahk3, and WT/ahk4). We then analyzed the vegetative growth, CK concentrations, and transcriptomes of these grafted plants.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kota Monden

PROVIDER: E-MTAB-15416 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Grafting is a well-established practice for grapevine to facilitate propagation of productive and tolerant cultivars against several stress factors. It is also considered to be a suitable method for studying molecular aspects of root-to-shoot and/or shoot-to-root signaling events. So far, controlling only effect of rootstock over scion was investigated and root-to-shoot transcriptomic alterations were fallowed on the scions or graft interfaces. The objective of this study was to investigate transcriptomic and physiological influence of scion on the rootstock under drought stress. Therefore, drought tolerant 110R rootstock were firstly grafted with sultana seedless and tested under drought stress with its non-grafted counterpart. The results of treatment indicated that grafted 110R performed the highest root elongation under drought. We carried out a microarray based transcriptome analysis on the roots of grafted and non-grafted 110R to explain this drought derived interaction through scion-to-rootstock. The highest expression increase under drought was recorded for sugar (SWEET) and nitrate or di/tri-peptide (NRT1/ PTR FAMILY) transporter proteins. Expression level of these genes was more highly increased in grafted 110R than its non-grafted counterpart. This situation indicated their potential role in drought tolerance and scion/rootstock harmony. Overexpression of these transporters attributed to increased amount of released nutrient and nitrogen source from abscised leaves of sultana seedless under drought. Remobilization of these rich sources was suggested to chance transcriptomic response of rootstocks and enabled much better growth in grafted 110R. Other transcripts annotated to cell wall modification enzymes (chitinases), osmoregulator proteins (dehydrins, proline-glycine rich proteins) and secondary metabolites (stilbene synthase) were also more highly induced in grafted 110R. This is the first report indicating transcriptomic influence of scion on the grapevine rootstocks and representing the genes responsible in scion/rootstock harmony and drought tolerance.

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Dataset Information

Effects of root-specific deficiency of each cytokinin receptor AHK2, AHK3, or AHK4 on genome-wide gene expression in shoots and roots of Arabidopsis thaliana

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