RNA-seq in WTC11 i3 lower-motor neurons with and without TDP-43 knockdown

ABSTRACT: Nuclear depletion and cytoplasmic aggregation of the RNA-binding protein TDP-43 is the hallmark of ALS, occurring in over 97% of cases. A key consequence of TDP-43 nuclear loss is the de-repression of cryptic exons. Motor neurons are the vulnerable cell-type in ALS and cryptic exons show variability in expression by cell-type. A human induced pluripotent stem cell (iPSC) line (WTC11), stably expressing a doxycycline-inducible hNIL construct and CRISPR/Cas9 was used in this study. Differentiation of human iPSCs was performed as previously reported. Briefly, iPSC induction was achieved using induction medium containing DMEM/F12, GlutaMAX supplement medium (Thermo Fisher), MEM non-essential amino acids (Thermo Fisher), N2 supplement (Thermo Fisher), 0.2 mM compound E, 2 μg/ml doxycycline, Pen

INSTRUMENT(S): NA, NextSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Anna-Leigh Brown 

PROVIDER: E-MTAB-15433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress