Unknown,Transcriptomics,Genomics,Proteomics

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RNA sequencing of canine oral melanoma and matched peripheral blood pre- and post-radiotherapy


ABSTRACT: Canine oral melanoma is a highly aggressive tumor with limited therapeutic options and a poor prognosis. To elucidate the molecular mechanisms underlying its progression and response to radiation therapy (RT), we performed a longitudinal multi-omics analysis incorporating whole-genome sequencing (WGS) and RNA sequencing (RNA-seq) on tumor and blood samples from a single canine case. WGS was performed on tumor and blood collected before RT (Pre-RT) and on blood collected immediately after the final radiation fraction (Post-RT 0m). RNA-seq was performed on tumor and blood collected before RT (Pre-RT) and immediately after the final radiation fraction (Post-RT 0m), and on tumor collected 4 months after RT (Post-RT 4m).

INSTRUMENT(S): Tru-cut biopsy needle (Marquee®, 16 G × 10 cm), Illumina NovaSeq X, TruSeq Stranded Total RNA Library Prep Gold Kit, TruSeq Stranded Total RNA Globin Kit, .

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Seungwan Woo 

PROVIDER: E-MTAB-15590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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