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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of bone marrow derived macrophages from mice undergoing hind-limb ischaemia surgery versus controls with or without lipopolysaccharide stimulation

ABSTRACT: Bone marrow derived macrophages (BMDMs) were cultured from hind limb bone marrow (femur plus tibia) collected from C57Bl/6 mice 7-days after induction of unilateral (left) hind limb ischaemia by ligation of the femoral artery, along with control mice not undergoing surgery, providing 6 control mouse samples, 3 ischaemic limb samples and 3 non-ischaemic limb samples. Further BMDMs from these groups were also cultured with lipopolysaccharide (LPS) to explore the impact of bone marrow ischaemia on a pathogen-associated molecular pattern response, providing 6 control mouse LPS samples, 3 ischaemic limb LPS samples and 3 non-ischaemic limb LPS samples.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Richard Cubbon

PROVIDER: E-MTAB-15624 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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