Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of tost-1(xf196 ts) mutant and wild-type embryos at 15°C and 25°C


ABSTRACT: This study investigates the role of TOST-1 and the PETISCO complex in regulating histone mRNA stability during Caenorhabditis elegans oogenesis and early embryogenesis. We performed RNA-sequencing analysis on early embryos from tost-1(xf196 ts) temperature-sensitive mutants compared to wild-type controls to understand the molecular basis of TOST-1's essential role in embryonic development and its effects on gene expression timing during the maternal-to-zygotic transition. We examined how temperature affects gene expression, since the developmental phenotype is temperature-dependent: at the permissive temperature (15°C) embryos develop normally, while at the restrictive temperature (25°C) development arrests and embryos die, allowing us to distinguish primary TOST-1 functions from secondary effects of developmental failure.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Rene Ketting 

PROVIDER: E-MTAB-15730 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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