Notch signaling activation in hPSC-derived CNS-like endothelial cells induces differential expression of blood-brain barrier-related genes

ABSTRACT: Mechanisms guiding the induction of blood-brain barrier (BBB) properties in central nervous system (CNS) endothelial cells during human development are incompletely understood. To explore induction of reduced vesicular endocytosis and transcytosis properties in a human in vitro model of the BBB, we used human pluripotent stem cell (hPSC)-derived endothelial progenitor cells (EPCs) in which Wnt/β-catenin signaling was activated to generate hPSC-derived CNS-like ECs (hPSC-CECs). We assessed the effects of Notch signaling through overexpression of the Notch1 receptor intracellular domain (N1ICD). N1ICD overexpression in hPSC-CECs was induced by treatment with doxycycline (Dox), and hPSC-CECs were sorted into subpopulations by FACS based on high or low surface expression of CD144/VE-cadherin (

INSTRUMENT(S): Illumina NovaSeq 6000, Qubit 4 Fluorometer

ORGANISM(S): Homo sapiens

SUBMITTER: Sarah Boutom 

PROVIDER: E-MTAB-15954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress