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Transcriptomic profiling of AR knockdown in human triple-negative breast cancer Hs578T cells

ABSTRACT: This study aimed to investigate transcriptomic alterations induced by androgen receptor (AR) silencing in the radioresistant Hs578T triple-negative breast cancer (TNBC) cells. Using siRNA-mediated knockdown of AR, RNA sequencing was performed to identify genes and pathways associated with AR signaling in radioresistant TNBC cells. Comparative transcriptomic profiling between control and AR-silenced cells revealed significant changes in pathways related to DNA damage response, apoptosis, and mTOR signaling. These data provide insights into the molecular mechanisms underlying AR-mediated regulation in TNBC and potential targets for therapeutic intervention.

INSTRUMENT(S): Agilent 2100 Bioanalyzer; Illumina TruSeq Stranded mRNA Prep Kit, CO₂ incubator (Thermo Fisher Scientific), Illumina NovaSeq 6000, Illumina TruSeq Stranded mRNA Library Prep Kit, Biological safety cabinet (Thermo Fisher Scientific), Lipofectamine RNAiMax transfection reagent (Invitrogen)

ORGANISM(S): Homo sapiens

SUBMITTER: Minji An

PROVIDER: E-MTAB-16017 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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