Single-cell RNA sequencing of murine lung in steady state, after IL-33 treatment or HDM treatment

ABSTRACT: Single cell RNA sequencing of the lung in steady state, after 1 or 3 doses of IL-33 or after HDM treatment Exp1: steady state lung cells (both CD45- as CD45+) from 4 untreated mice. Cells from distinct replicates are labeled with unique HTO barcodes. Exp2: lung cells (both CD45- as CD45+) from 2 mice (PBS intratracheal treatment), 4 mice (1xIL33 intratracheal treatment) and 4 mice (3 x IL33 intratracheal treatment). Cells from distinct replicates are labeled with unique HTO barcodes. Exp3: lung, bone marrow and blood cells (CD45+ CD3- CD19-) from 3 mice (House dust mite intratracheal (HDM) treatment and 14 days later PBS intranasal treatment on 4 consecutive days) and 3 mice (HDM treatment and 14 days later HDM intranasal treatment on 4 consecutive days). Mice were pooled and different or

INSTRUMENT(S): 10X Chromium, FACS BD Aria II & III, Illumina NovaSeq 6000, Cellranger

ORGANISM(S): Mus musculus

SUBMITTER: Stijn Verwaerde 

PROVIDER: E-MTAB-16099 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress