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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of human pancreas organoids in expansion conditions in the presence of DMSO, CHIR99021 or CHIR99021 in the absence of FGF2

ABSTRACT: To investigate signaling pathways regulating human pancreas differentiation and morphogenesis, we developed a high-content image-based screen and quantitative multivariate analysis pipelines robust to heterogeneity to extract single-cell and organoid features using pancreatic progenitor organoids. Among the 54 hit compounds, we found that GSK3A/B inhibition via WNT signaling has a global reversible effect on cell identity, repressing pancreatic progenitor markers and inducing a poised state of progenitors transitioning to acinar cells. We show that additional FGF repression enables further differentiation of acinar cells recapitulating pancreatic acini morphogenesis and functions . This dataset compares human pancreas organoids produced from pluripotent stem cells by RNA-Seq in expansion conditions in the presence of DMSO (control) to those exposed for 7 days to CHIR99021 or CHIR99021 in the absence of the FGF2 normally included in the expansion medium.

INSTRUMENT(S): Cell culture, Chromium Controller, 10x Genomics and Chromium X, 10x Genomics, Chromium Controller, 10x Genomics and Chromium X, 10x Genomics, Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Anne Grapin-Botton

PROVIDER: E-MTAB-16105 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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