Pro-tumorigenic effects of HLA-G in Renal Cell Carcinoma

ABSTRACT: Renal cell carcinoma (RCC) frequently exhibits high expression of the immunomodulatory molecule HLA-G, which promotes immune tolerance and tumor progression. To define the cell-intrinsic transcriptional programs driven specifically by HLA-G1 neo-expression, we generated RCC7/HLA-G1 cells by lentiviral overexpression and compared them with parental RCC7wt cells that lack endogenous HLA-G. Phenotypically, RCC7/HLA-G1 cells display increased proliferation, enhanced migratory capacity, resistance to apoptosis, and accelerated tumor growth in vivo. The purpose of this bulk RNA-sequencing experiment is to characterize the global transcriptional changes induced by HLA-G1 and to identify signaling pathways, gene networks, and regulatory modules that underlie its pro-tumorigenic activity. By compar

INSTRUMENT(S): Genomics Core Shared Resources at Augusta University (RRID: SCR_026483), gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec, Cat. No. 130-096-427) together with the Human Tumor Dissociation Kit (Miltenyi Biotec, Cat. No. 130-095-929),, Illumina NovaSeq 6000, RNeasy Mini Kit (Qiagen)

ORGANISM(S): Homo sapiens

SUBMITTER: Ashwin Ajith 

PROVIDER: E-MTAB-16395 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress