PKC-dependent MYRF dysregulation links Aβ pathology to oligodendrocyte, myelin and cognitive alterations in Alzheimer’s disease

ABSTRACT: Alzheimer’s disease (AD) is characterized not only by neuronal loss and synaptic dysfunction but also by emerging evidence of oligodendrocyte and myelin pathology. Amyloid-β (Aβ), a hallmark of AD, disrupts oligodendrocyte homeostasis through mechanisms that remain poorly understood. Here, we investigated the role of the myelin regulatory factor (MYRF), a transcription factor essential for oligodendrocyte maturation, in AD-related glial dysfunction. Using the 3xTg-AD mouse model, we observed aberrantly induced maturation dynamics and reduced oligodendrocyte lineage cell density at 12 months in the dentate gyrus, accompanied by increased MYRF expression. Interestingly, sustained MYRF overexpression was found to be toxic for oligodendrocytes in vitro. Moreover, in vitro and in vivo experimen

INSTRUMENT(S): Single Cell/Low Input RNA Library Prep Kit for Illumina (#E6420S, New England Biolabs), STAR, MACS, Illumina HiScanSQ, RNA Micro Kit (Qiagen).

ORGANISM(S): Mus musculus

SUBMITTER: Rodrigo Senovilla-Ganzo 

PROVIDER: E-MTAB-16400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress