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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq transcriptomics of doxorubicin (DOX) exposure in mouse colon tissue (in vivo; 0, 5, 10 mg/kg; 6, 24, 72, 96 h), with raw paired-end FASTQ files.

ABSTRACT: Doxorubicin (DOX) is a widely used chemotherapeutic agent that can cause off-target gastrointestinal (GI) toxicity. This experiment profiles DOX-induced transcriptomic responses by RNA-seq in mouse colon tissue (in vivo) across dose and time. Mice received DOX at 5 or 10 mg/kg by intravenous bolus injection on days 0 and 1, and were euthanized at 6, 24, 72, or 96 h after treatment initiation (the 6 h group was sampled prior to the second dose). Vehicle controls were dosed on the same schedule. Colon mucosal samples were collected for transcriptomic profiling, and raw paired-end FASTQ files are provided to support downstream analysis of GI toxicity mechanisms in vivo. A matched mouse colonoid (in vitro) RNA-seq dataset is deposited separately and can be linked via the related accession field and/or referenced in the manuscript.

INSTRUMENT(S): Illumina HiSeq 2500, Standard molecular biology equipment (per Lexogen SENSE mRNA-seq kit protocol), Tissue Homogenizer (required to process mucosal tissue for the miRNeasy kit). Refrigerated Centrifuge (4 °C) (required for the miRNeasy protocol)., NanoDrop ND-1000 spectrophotometer; Agilent 2100 Bioanalyzer

ORGANISM(S): Mus musculus

SUBMITTER: Saad Salman Khan Lodhi

PROVIDER: E-MTAB-16565 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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