Oxygen-dependent osteocyte transcriptomic adaptations occur without modulation of endothelial gene expression via osteocyte paracrine signaling

ABSTRACT: Bone regeneration remains clinically challenging, partly due to limited knowledge of the cellular mechanisms governing bone repair. Osteocytes, which constitute the majority of bone cells, form an dendritic network and are central regulators of skeletal homeostasis. Residing in a mineralized and low-oxygen environment, they are exposed to hypoxic conditions that may shape their function, yet the role of hypoxia in osteocyte behavior and angiogenic signaling during bone regeneration is still unclear. This study investigated the effects of hypoxia on osteocytes and their paracrine interaction with endothelial cells. MLO-Y4 osteocytic cells were cultured under normoxia (21% O₂), moderate hypoxia (5% O₂), or severe hypoxia (1% O₂), and conditioned media were applied to mouse endothelial cells.

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Frédéric Martins 

PROVIDER: E-MTAB-16618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress