Transcriptomics,Genomics

Dataset Information

28

Comparison of osteocyte-enriched bone from DMP1 cre Mbtps1 knockout mice with littermate controls.


ABSTRACT: Purpose of arrays were to determine what the effect of deletion of Mbtps1 gene was on gene expression of osteocytes in bone in vivo. DMP1 cre driver was used to delete the Mbtps1 gene in osteocytes and osteoblasts in bone. We then isolated osteocyte enriched bone particles from 40 week old male mice to determine the effect of this deletion on gene expression. We have previously shown that Mbtps1 is needed for transcription of Phex, DMP1, and MEPE genes in osteoblasts in culture. Arrays showed these genes were reduced as expected in osteocytes in vivo. Controls represent osteocyte enriched bone from 40 week old littermates. Also, as expected, Mbtps1 expression was reduced in these knockout mice Overall design: 4 control mice and 3 knock out mice were used in the analysis

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: Sridar V Chittur  

PROVIDER: GSE69975 | GEO | 2015-12-29

SECONDARY ACCESSION(S): PRJNA287321

REPOSITORIES: GEO

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Publications

Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age.

Gorski Jeff P JP   Huffman Nichole T NT   Vallejo Julian J   Brotto Leticia L   Chittur Sridar V SV   Breggia Anne A   Stern Amber A   Huang Jian J   Mo Chenglin C   Seidah Nabil G NG   Bonewald Lynda L   Brotto Marco M  

The Journal of biological chemistry 20151230 9


Conditional deletion of Mbtps1 (cKO) protease in bone osteocytes leads to an age-related increase in mass (12%) and in contractile force (30%) in adult slow twitch soleus muscles (SOL) with no effect on fast twitch extensor digitorum longus muscles. Surprisingly, bone from 10-12-month-old cKO animals was indistinguishable from controls in size, density, and morphology except for a 25% increase in stiffness. cKO SOL exhibited increased expression of Pax7, Myog, Myod1, Notch, and Myh3 and 6-fold m  ...[more]

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