Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Single-cell RNA sequencing of multi-lineage differentiating stress-enduring (Muse) cells in the naïve state and after phagocytosis of cardiomyocyte-, neural cell-, or hepatic cell–derived fragments

ABSTRACT: Stem cells undergo cytokine-driven differentiation, but this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing 'model cells' (apoptotic differentiated cells) was found to require only a short time frame. Pluripotent-like Muse cells phagocytosed apoptotic differentiated cells via different phagocytic receptor subsets than macrophages. The phagocytosed-differentiated cell-derived contents (e.g., transcription factors) were quickly released into the cytoplasm, translocated into the nucleus, and bound to promoter regions of the stem cell genomes. Within 24 ~ 36 h, the cells expressed lineage-specific markers corresponding to the phagocytosed-differentiated cells, both in vitro and in vivo. This scRNA-seq experiment aimed to elucidate the transcriptional changes induced by the phagocytosis of dead cell fragments derived from differentiated cells, including cardiomyocytes, neural cells, and hepatocytes.

INSTRUMENT(S): BD Rhapsody, Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: SHOHEI WAKAO

PROVIDER: E-MTAB-16736 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame.

Wakao Shohei S Oguma Yo Y Kushida Yoshihiro Y Kuroda Yasumasa Y Tatsumi Kazuki K Dezawa Mari M

Cellular and molecular life sciences : CMLS 20221006 11

Stem cells undergo cytokine-driven differentiation, but this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing 'model cells' (apoptotic differentiated cells) was found to require only a short time frame. Pluripotent-like Muse cells, multipotent mesenchymal stem cells (MSCs), and neural stem cells (NSCs) phagocytosed apoptotic differentiated cells via different phagocytic receptor subsets than macrophages. The phag ...[more]

PMID: 36203068

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Project description:The paper describes a model of effect of cellular de-differentiation on the dynamics and evolution of tissue and tumor cells. Created by COPASI 4.25 (Build 207) This model is described in the article: Effect of cellular de-differentiation on the dynamics and evolution of tissue and tumor cells in mathematical models with feedback regulation Dominik Wodarz J Theor Biol. 2018 July 07; 448: 86–93 Abstract: Tissues are maintained by adult stem cells that self-renew and also differentiate into functioning tissue cells. Homeostasis is achieved by a set of complex mechanisms that involve regulatory feedback loops. Similarly, tumors are believed to be maintained by a minority population of cancer stem cells, while the bulk of the tumor is made up of more differentiated cells, and there is indication that some of the feedback loops that operate in tissues continue to be functional in tumors. Mathematical models of such tissue hierarchies, including feedback loops, have been analyzed in a variety of different contexts. Apart from stem cells giving rise to differentiated cells, it has also been observed that more differentiated cells can de-differentiate into stem cells, both in healthy tissue and tumors, aspects of which have also been investigated mathematically. This paper analyses the effect of de-differentiation on the basic and evolutionary dynamics of cells in the context of tissue hierarchy models that include negative feedback regulation of the cell populations. The models predict that in the presence of de-differentiation, the fixation probability of a neutral mutant is lower than in its absence. Therefore, if de-differentiation occurs, a mutant with identical parameters compared to the wild-type cell population behaves like a disadvantageous mutant. Similarly, the process of de-differentiation is found to lower the fixation probability of an advantageous mutant. These results indicate that the presence of de- differentiation can lower the rates of tumor initiation and progression in the context of the models considered here. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Single-cell RNA sequencing of multi-lineage differentiating stress-enduring (Muse) cells in the naïve state and after phagocytosis of cardiomyocyte-, neural cell-, or hepatic cell–derived fragments

Publications

Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame.

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