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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA seq of natural killer (NK) cells of MPN patients with mutations in calreticulin (CALR) and healthy controls

ABSTRACT: To assess the transcriptional differences between natural killer (NK) cells from patients with myeloproliferative neoplasms (MPN) carrying a mutation in calreticulin (CALR) and healthy donors (HD), bulk RNA sequencing was performed. Peripheral blood mononuclear cells (PBMC) were isolated from the blood of 4 HD and 4 CALR-mutant MPN patients using Ficoll density gradient and were cryopreserved after the isolation process. CD45+SSClowCD3-CD56+ cells were sorted from cryopreserved PBMC samples and RNA was isolated.

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Homo sapiens

SUBMITTER: Agnieszka Witalisz-Siepracka

PROVIDER: E-MTAB-16774 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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