Project description:Whole blood total RNA was isolated form PaxGene tubes using the PaxGene total RNA extraction kits. miRNA expression was profiled in Primary Sjogren's Syndrome patients and controls in order to independently validate differentially expressed miRNAs between patients and controls and between patient subgroups that were identified in 'Cohort 1'.
Project description:Whole blood total RNA was isolated form PaxGene tubes using the PaxGene total RNA extraction kits. miRNA expression was profiled in Primary Sjogren's Syndrome patients and controls in order to independently validate differentially expressed miRNAs between patients and controls and between patient subgroups that were identified in Cohorts 2 and 3.
Project description:Cardiomyocytes derived from human pluripotent stem cells were exposed to the cardiotoxic drug Doxorubicin in order to assess the utility of this cell system as a model for drug-induced cardiotoxicity. Cells are exposed to different concentrations of doxorubicin for up to 48 hours followed by a 12 days recovery period.
Project description:Whole blood total RNA was isolated form PaxGene tubes using the PaxGene total RNA extraction kits. miRNA expression was profiled in Primary Sjogren's Syndrome patients and controls in order to identify differentially expressed miRNAs between patients and controls and between patient subgroups.
Project description:microRNAs were profiled in healthy controls, classic celiac patients (CD), CD patients with anemia and GFD treated CD with normalization of duodenal mucosa all CD conditions were related to controls. For each group, five patients were pooled. One replicate per experiment
Project description:Endothelial-enriched total RNAs were obtained from the suprarenal region of the abdominal aorta which is the murine AAA prone area in AngII-infused C57BL/6 mice. At 12 or 36h post-AngII pump implantation, endothelial-enriched RNAs from four abdominal aortas were pooled to obtain ~30 ng total RNA as one array sample, performed in triplicates. All RNA samples used for miRNA microarray study passed the initial quality control test and each sample was linearly amplified.
Project description:We report the identification of microRNA-138 (miR-138), as a molecular signature of GSCs and demonstrate a vital role for miR-138 to promote growth and survival of bona fide tumor-initiating cells with self-renewal potential. Total RNA from Glioma Stem Cells and Neural Stem Cells were subjected to microRNA microarray analysis, 3 replicates each.
Project description:We performed global microRNA expression profiling of a cohort of primary melanoma patient samples linked to a well-annotated clinical database. The goal of this study was to identify microRNA that are associated to or correlated with various clinical parameters and patient outcomes. Candidate microRNA were identified for building prognostic models and functional testing. 119 primary melanoma samples were analyzed in two color arrays. The reference sample used was an equal combination of all samples analyzed. By this design, ratio data of test sample/reference is mean-centered data.