CUT&RUN LoV-U time-course profiling of Wnt transcriptional complex in ΔCTNNB1 HEK293T cells compared to wild-type

ABSTRACT: Wnt/β-catenin signaling is commonly understood to activate transcription through pre-accessible regulatory elements. To investigate whether this pathway also directly modulates chromatin states, we performed CUT&RUN in human embryonic kidney 293T (HEK293T) and matched ΔCTNNB1 cells to profile β-catenin-associated chromatin complexes following pathway activation. Cells were pre-treated with the PORCN inhibitor LGK974 (10 nM) for 24 hours and subsequently stimulated with the GSK3 inhibitor CHIR99021 (10 µM) for 30 minutes, 90 minutes, or 4 hours to activate Wnt/β-catenin signaling. Chromatin binding of β-catenin, pan-TCF/LEF (LEF1, TCF7, TCF7L1, and TCF7L2), CBP/p300, HDAC1, H3K27ac, and H3K4me1 was assessed across conditions. An IgG control was included for all experiments. For perturbatio

INSTRUMENT(S): KAPA EvoPrep Kit, Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Tamina Weiss 

PROVIDER: E-MTAB-17186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress