Spatial transcriptomic profiling of quadriceps muscles from mice fed either standard diet or high-fat diet for 20 weeks
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ABSTRACT: Intermuscular adipose tissue (IMAT) accumulation within skeletal muscle is associated with cardiometabolic disease, yet its spatial organization and molecular drivers remain poorly characterized. To map IMAT expansion within the quadriceps muscle architecture, spatial transcriptomics was performed on tissue sections from diet-induced obese male C57BL/6J mice fed a 60% kcal high fat diet (HFD) or 10% kcal low fat diet (LFD) for 20 weeks. The 10x Genomics Visium platform was used to generate spatially resolved gene expression profiles, which were integrated with immunofluorescence staining for Perilipin (adipocyte marker) and Laminin (muscle fiber boundaries) to relate transcriptional signatures to tissue morphology. This dataset enables spatially resolved investigation of gene expression changes associated with IMAT expansion in the context of diet-induced obesity.
INSTRUMENT(S): Not applicable, Illumina NovaSeq X
ORGANISM(S): Mus musculus
SUBMITTER: Dominik Lutter
PROVIDER: E-MTAB-17271 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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