ChIP-seq profiling of histone modifications (H3K4me3, H3K9ac, and H3K27me3) in seedling shoot tissues from 10 barley genotypes
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ABSTRACT: ChIP-seq was performed to characterize histone modification landscapes and to enable comparative epigenomic analyses across the barley pangenome. Leaf tissues from seedling shoots of 10 barley genotypes were collected from plants grown under controlled conditions (16 h light/8 h dark, 20°C day/16°C night). Chromatin was crosslinked with 1% formaldehyde, quenched with glycine, and nuclei were isolated following tissue homogenization. Chromatin was sheared by focused ultrasonication and immunoprecipitated using antibodies specific to H3K4me3, H3K9ac, and H3K27me3. ChIP and input DNA libraries were sequenced as paired-end reads (2 × 151 bp) on Illumina NovaSeq platforms, with 2–3 biological replicates per genotype per histone mark.
INSTRUMENT(S): Illumina NovaSeq 6000
ORGANISM(S): Hordeum vulgare subsp. spontaneum
SUBMITTER: Zihao Zhu
PROVIDER: E-MTAB-17344 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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