RNA-seq of NLL cv. Oskar from 12 in-house leaf and stem control and RAP 2-7 mutant samples

ABSTRACT: Total RNA was isolated from 100 mg of powdered tissue using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich), which included on-column DNAse treatment to remove genomic DNA contamination. The purified RNA was flash-frozen and dispatched to Macrogen (Seoul, Korea) for library preparation and sequencing. Following quality control checks, sequencing libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) as per the manufacturer's protocol (Part #15031047 Rev. E). The libraries were sequenced on an Illumina platform, producing 151-bp paired-end reads, with each sample yielding between 64–87 million paired-end reads.

INSTRUMENT(S): Illumina NovaSeq 6000, Illumina

ORGANISM(S): Lupinus angustifolius

SUBMITTER: Jooa Moon 

PROVIDER: E-MTAB-17708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress