Unknown,Transcriptomics,Genomics,Proteomics

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MNASE_seq_of_AGO2_Knock_Down_HeLaS3_cells

ABSTRACT: We knocked down AGO2 protein by RNAi in HeLaS3 human cell line. The current experiment aims at comparing nucleosome positioning in control and AGO2 knock down cells. HeLaS3 cell were cultured in DMEM. Cells were transfected with AGO2 siRNAs (or control siRNA) at day 1. On day 4 MNAse digestion of chromatin was performed. Mnase-digested chromatin DNA fragments were sequenced by paired-end DNA-seq.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Homo sapiens

SUBMITTER: Valerio Fulci

PROVIDER: E-MTAB-1844 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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