Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Arabidopsis_thaliana_floral_meristem_atlas

ABSTRACT: Transcriptomic changes during early developmental stages of reproductive meristems in Arabidopsis thaliana. Through laser microdissection coupled to RNA sequencing, we provide precise transcriptomic analysis of the inflorescence meristem, floral meristem and of the flower at the beginning of organ differentiation. Transcript abundance quantification and differential expression (DE) analyses were performed using the Bowtie/Tophat/Cufflinks pipeline (BTC) (Trapnell et al., 2012) with the RefSeq Arabidopsis annotation. The BTC pipeline identified 46 genes as differentially expressed between IM and FM, 171 genes differentially expressed between FM and ST3 and 178 genes differentially expressed between IM and ST3. The obtained data turned out to be solid since they are highly consistent with established knowledge but they also allowed the identification of new high profile candidate genes as well as new insights into potential regulatory networks underlying the formation of these reproductive structures. We provide a web interface for easy interrogation of these unique datasets.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Matteo Chiara 

PROVIDER: E-MTAB-1946 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene coexpression patterns during early development of the native Arabidopsis reproductive meristem: novel candidate developmental regulators and patterns of functional redundancy.

Mantegazza Otho O   Gregis Veronica V   Chiara Matteo M   Selva Caterina C   Leo Giulia G   Horner David S DS   Kater Martin M MM  

The Plant journal : for cell and molecular biology 20140728 5

During very early stages of flower development in Arabidopsis thaliana, a series of key decisions are taken. Indeed, the position and the basic patterning of new flowers are determined in less than 4 days. Given that the scientific literature provides hard evidence for the function of only 10% of A. thaliana genes, we hypothesized that although many essential genes have already been identified, many poorly characterized genes are likely to be involved in floral patterning. In the current study,  ...[more]

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