GDF5 variant with superior healing capacity

ABSTRACT: Seventy-two female Sprague-Dawley rats underwent a 5 mm critical-sized femoral segmental defect followed by stabilization with a custom-made unilateral external fixator. Following femoral defect generation, absorbable type I bovine collagen sponges were loaded either with 50 ug GDF5wt or 50 ug GDF5N445T, dissolved in dilution buffer (10 mM HCl), and placed into the defect. The control group was treated with collagen sponges soaked with dilution buffer. For analysis of the early healing phase, 48 rats (n = 4 rats/group & time point) were sacrificed at day 3, 5, 7 and 14 post-operation and the callus tissue between the two inner pins was harvested and immediately flash frozen in liquid nitrogen for RNA extraction. Tissue was subsequently pulverized and total RNA was isolated using Trifast re

ORGANISM(S): Rattus norvegicus

SUBMITTER: Claus Ott 

PROVIDER: E-MTAB-2249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress