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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide analysis of Groucho in Drosophila Kc167 and S2R+ cell lines

ABSTRACT: In this study we have used ChIP followed by high throughput sequencing to profile the genome-wide recruitment of wildtype and non-oligomerizing Groucho (Gro) at high resolution in single cell types (Kc167 and S2R+) using Drosophila cell culture. Our results reveal that Gro is typically recruited to discrete peaks in active chromatin and that blocking Gro oligomerization does not change the width of the peaks to which it is recruited. We have also investigated acetylated histone H3 and H4 and RNA polymerase II profiles around Gro binding sites, along with gene expression, in wildtype, Gro knockdown, Gro-GFP transfected, and non-oligomerizing Gro-GFP transfected Kc167 cells and found that Gro associates with chromatin containing hypoacetylated histones and frequently overlaps the transcription start sites of expressed genes that exhibit strong RNA polymerase pausing.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Aamna Kaul

PROVIDER: E-MTAB-2316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Groucho co-repressor is primarily recruited to local target sites in active chromatin to attenuate transcription.

Kaul Aamna A Schuster Eugene E Jennings Barbara H BH

PLoS genetics 20140828 8

Gene expression is regulated by the complex interaction between transcriptional activators and repressors, which function in part by recruiting histone-modifying enzymes to control accessibility of DNA to RNA polymerase. The evolutionarily conserved family of Groucho/Transducin-Like Enhancer of split (Gro/TLE) proteins act as co-repressors for numerous transcription factors. Gro/TLE proteins act in several key pathways during development (including Notch and Wnt signaling), and are implicated in ...[more]

PMID: 25165826

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