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Pioglitazone competes with EGR1 to suppress PKD1 in Angiotensin II-treated macrophages

ABSTRACT: THP-1 cells were differentiated to macrophages with the phorbol 12-myristate 13-acetate (PMA) for a total time of 28 hours. To understand the effects of Angiotensin II (AngII) and of AngII in the presence of the thiazolidinedione pioglitazone (Pio), after 4 hours of PMA-induced differentiation of THP-1 cells (when cells became adherent), the PMA-containing medium was supplemented with AngII (1mg/ml), AngII (1 mg/ml) with Pio (1 uM), or no supplement for another 24 hours. At this time point the cells were harvested, RNA was extracted and used for a microarray-based gene profiling analysis of THP-1 cells, under the three conditions: PMA, AngII and AngII with Pio.

ORGANISM(S): Homo sapiens

SUBMITTER: Ken Laing

PROVIDER: E-MTAB-2320 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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