Comprehensive transcriptome analysis of pig induced pluripotent stem cells by high-throughput sequencing

ABSTRACT: The wide application of pig disease model has caused a surge of interest in the study ofderivation of pig induced pluripotent cells (iPSCs). Here we performed genome-wide analysis of gene expression profiling by RNA-seq and small RNA-seq and DNA methylation profile by MeDIP-seq in pig iPSCs through comparison with somatic cells. We identified mRNA and microRNA transcripts that were specifically expressed in pig iPSCs. Our analysis identifies the genes up-regulated in pig iPS compared with somatic cells and also the differentially expressed genes between pig iPSCs under different culture medium. We then pursued comprehensive bioinformatics analyses, including functional annotation of the generated data within the context of biological pathways, to uncover novel biological functions associat

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Sus scrofa

SUBMITTER: Liu Yajun 

PROVIDER: E-MTAB-2634 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress