Transcription profiling of pigment cells in the tunicate Ciona intestinalis to study pigment cell specification within Central Nervous System

ABSTRACT: Its known that FGF signaling induces pigment cell precursors formation. We perturbed the endogenous FGF signaling cascade by using a dominant-negative form of the unique Ci-FGF receptor (FGFRDN) and a constitutively active form of Ci-Ets1/2 (Ets:Vp16), a transcriptional effector of FGF/MAP Kinase cascade. Targeted expression of dominant negative FGF receptor and Ets:VP16 fusion protein was achieved in PCPs using the Tyrosinase-related protein 1/2a (Tyrp1/2a) cis-regulatory region. The PCPs were isolated by Fluorescent Activated Cell Sorting (FACS) as GFP+ cells at two developmental stages: 8 hpf at ~16C (neurula stage) corresponding to early FGF-mediated induction and 12 hpf at ~18C (tailbud stage) where PCPs are already fate restricted as pigment cells. To avoid a contamination by mesench

ORGANISM(S): Ciona intestinalis

SUBMITTER: Claudia Racioppi 

PROVIDER: E-MTAB-2759 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress