Transciption profiling by array of human umbilical cord blood Natural Killer cells after overnight treatment with or without IL-15

ABSTRACT: Cord blood NK cells were cultured with or without IL-15 overnight and then RNA was isolated using an RNeasy Mini kit (Qiagen). Samples were processed using GeneChip Whole Transcript Sense Target Labeling assays using the Ambion WT Expression kit and Affymetrix GeneChip WT Terminal Labeling and Controls kit (Affymetrix). The resulting ssDNAs were hybridized to the Human Gene 2.0 ST Array (Affymetrix) and transcript levels compared between IL-15 treated and untreated using GeneSpring software.

ORGANISM(S): Homo sapiens

SUBMITTER: Aurore Saudemont 

PROVIDER: E-MTAB-2847 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress