Single-cell RNA-sequencing of Platynereis dumerilii larval brain cells

ABSTRACT: Understanding cell type identity in complex tissues or organisms requires integration of each cell's expression profile with its spatial location within the tissue under study. We developed a high-throughput method that combines in vitro single-cell RNA-sequencing with a gene expression atlas to map single cells back to their location within the tissue of interest. We used the developing brain of a marine annelid, Platynereis dumerilii that is an important model system for studying bilaterian brain evolution, to benchmark our approach. To generate the single-cell mRNA-sequencing data, P. dumerilii larval brains were dissociated, followed by cell capture, cDNA synthesis and amplification on the C1 Single-Cell Auto Prep IFC for 10-17 um cells (Fluidigm). Sequencing libraries were produced us

INSTRUMENT(S): C1 Single-Cell Auto Prep System, Fluidigm, Illumina HiSeq 2000

ORGANISM(S): Platynereis dumerilii

SUBMITTER: John Marioni 

PROVIDER: E-MTAB-2865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress