Identification of candidate non-host resistance genes for powdery mildew in barley

ABSTRACT: To identify the non-host responsive genes, a time-series based global expression profiling was performed in barley using Agilent chip. Seven-day-old barley plants of cv. Vada were inoculated with spore density of 50-80 conidia mm-2 of Bgh or Bgt, and the abaxial epidermis of inoculated primary leaves or from non-inoculated control leaves was peeled 6, 12, 24 and 74 h after inoculation. Total RNA was extracted by using the RNeasy plant mini kit with on-Column DNase digestion (Qiagen, Hilden, Germany), and hybridized to a 44K Agilent oligonucleotide custom array of barley.

INSTRUMENT(S): Agilent G2505B scanner

ORGANISM(S): Hordeum vulgare

SUBMITTER: Jeyaraman Rajaraman 

PROVIDER: E-MTAB-2916 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress