Differential DNA methylation profiles of coding and non-coding genes define hippocampal sclerosis in human temporal lobe epilepsy

ABSTRACT: Methylation profiling was carried using Methylated DNA immunoprecipitation coupled with CpG island plus promoter array for human tissue samples. The samples, i.e. immunoprecipitate & input after immunoprecipitation were deferentially labelled using Cy5 & Cy3 flourophores. These were subsequently hybridized on the arrays and thereafter washed and scanned. The samples used for this study Hippocampal tissue was scored according to the Watson hippocampal sclerosis grading system- Watson Grade 1 or 4. Control tissue (labelled as Control in disease staging) was obtained from the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD.

INSTRUMENT(S): Scanning of was performed on an Axon 4000B scanner

ORGANISM(S): Homo sapiens

SUBMITTER: Sudipto Das 

PROVIDER: E-MTAB-3054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress